Törer, Hakan, Elif Burcu Aydın, and Mustafa Kemal Sezgintürk. Analytica chimica acta 1024 (2018): 65-72.
An ultrasensitive electrochemical immunosensor for RACK 1 detection uses 11-cyanoundecyltrimethoxysilane (11-CUTMS) as an immobilization matrix to immobilize the biorecognition element. The silane agent (11-CUTMS) used was efficient for the loading of anti-RACK 1 antibodies. The immunosensor was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM) images, and Fourier transform infrared spectroscopy (FTIR) spectroscopy. Electrochemical characterization The immunosensor was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. In addition, the biorecognition interaction between the anti-RACK1 antibody and the RACK1 antigen was monitored using the single frequency technique (SFI). Due to the high specificity, good reproducibility, and long stability due to reusability, the fabricated immunosensor will provide a sensitive and selective method for RACK 1 detection.
In the first step of the study, the CUTMS concentration for SAM formation was optimized. This step is necessary to bind a sufficient amount of the biorecognition molecule (anti-RACK 1 antibody) to the electrode surface. Therefore, three different concentrations (0.1%; 0.5%; 1% w/w) were tried. CUTMS has two groups; one end is a silane group and the other end is a nitrile group. These silane ends bind to the ITO electrode after the hydroxylation treatment of the ITO electrode. Using 0.1% concentration of CUTMS was not enough to form a SAM on the ITO electrode. Using 1% concentration of 11-CUTMS caused deterioration, so a normal signal could not be obtained. After obtaining the best signal, 0.5% concentration of CUTMS was used.
Özcan, Burcu, and Mustafa Kemal Sezgintürk. International journal of biological macromolecules 130 (2019): 245-252.
